Fred David Mast

Areas of expertise: systems cell biology; evolutionary cell biology; biochemistry; molecular biology; high-throughput data analysis. Specialties: microscopy - live and fixed cell quantitative and automated fluorescence imaging; flow cytometry; proteomics - automated affinity capture of protein and nucleic acid complexes; in vitro biochemical assays; fractionation and enrichment of subcellular organelles, compartments and complexes; structure-function mapping; functional genomics; yeast biology; mammalian cell culture; Drosophila; gene silencing - RNAi, shRNA; flavivirus (Dengue and Zika) propagation and infectivity analyses; Mycobacterium tuberculosis; bioinformatics; phylogenetic analysis - Bayesian, maximum likelihood, cladistics; pattern recognition, clustering and classification