The scientific activity of Margherita Ruoppolo has focused on the structural characterization of proteins and metabolites and is traceable to three essential themes: Characterization of post-translational modifications with particular regard to the dynamic characterization of disulfuric bridges and to oxidative folding processes. Development of differential and functional proteomics methodologies in molecular medicine Development of metabolomics methodologies in the study of inborn errors of metabolism Currently her research concerns the definition of altered intracellular pathways in hereditary diseases of the intermediate metabolism. She is the author of 70 publications in international journals.