Matthias Lutolf (born in 1973, also known as Matthias Lütolf) is a bio-engineer and a professor at EPFL (École Polytechnique Fédérale de Lausanne) where he leads the Laboratory of Stem Cell Bioengineering. He is specialised in biomaterials, and in combining stem cell biology and engineering to develop improved organoid models.

Lutolf's laboratory develops in vitro organoids mimicking healthy and diseased tissues and organs. Specifically, Lutolf uses bioengineering strategies to guide stem cell-based development to build novel organoids with improved reproducibility and physiological relevance for basic science and in vitro testing of drug candidates. His team has developed approaches to generate organoids in fully controllable 3D matrices, and has contributed to the understanding of how extrinsic biochemical and physical factors control stem cell fate and organogenesis. His team has developed concepts based on microfabrication, bioprinting, and microfluidics to improve the reproducibility, size, shape, and function of organoids.