RED LIGHT IRRADIATION REGULATES ROS SCAVENGING AND ANTI-INflAMMATION THROUGH SPHK1/NF-ΚB PATHWAY IN HACAT CELLS

Objectives Oxidative stress is a well-accepted pathogenesis of several human diseases, which is an increased amount of the oxidants exceeding the capacity of antioxidant defense system. Light-emitting diode irradiation (LEDI) represents an efficient strategy to counteract this condition. The purpose of the present study was to evaluate the ROS scavenging and anti-inflammatory mechanism of LEDI. Findings cDNA microarray, semi quantitative PCR (semi-qPCR), western blotting and small-interfering RNA (siRNA) transfection were processed on PMA induced oxidative stress and inflammation in HaCaT cells. In this study, 625 nm LEDI showed the effect of ROS scavenging and anti-inflammation. One of the most important genes which identified by microarray analysis was sphingosine kinase-1 (SPHK1), which is a key enzyme in sphingolipid metabolism. SPHK1 knockdown drastically reduced the viability of ROS scavenging in the presence of PMA-stimulated HaCaT cells. Furthermore, results with cyclooxygenease-2 (COX-2) and prostaglandin E2(PGE2) further indicated the importance of the SPHK1 in anti-inflammatory process in HaCaT cells. Conclusions The results obtained in this work highlight the possible role of SPHK1 in ROS scavenging and anti-inflammation in PMA-stimulated HaCaT cells, investigating for the first time the possibility its involved molecular mechanisms. And SPHK1 can be used as a therapeutic target in LEDI treatments for treating skin disorders through ROS scavenging and/or anti-inflammation.