Cryo-EM Information Management System and Sample Evaluation at Stanford-SLAC Cryo-EM Center

The Stanford-SLAC Cryo-EM Center (S 2 C 2 ) [1] is a national service center funded under the NIH Transformative High-Resolution Cryoelectron Microscopy Program. Our mission is to provide access to high resolution cryo-EM data collection and training for biomedical research. We have implemented a data management workflow from cryo-specimen preparation to data collection using a Cryo-EM Information Management System (CiMS) eLogBook. The eLogbook is a data management software platform used in multiple facilities at SLAC, providing a file catalog, laboratory notebook, sample management and other functionality. Its composable architecture supports pluggable components to enable features specific to the cryo-EM workflow. A Python based web services application programming interface allows users to record experimental meta data as well as automation that seamlessly transfers large volumes of data. It enables users to manage experiments, provides a web-based interface to automatically move data from microscopes to the data center, sets up permission to the data, manages remote access to microscopes via FastX, provides real-time previews of motion-corrected images and corresponding contrast transfer functions (CTF) via Slack messaging app, provides summary information from MotionCorr2 [2] and CTFFIND4 [3] programs on a web browser, and provides notebook functionality to record experiments. In addition, it also manages data subsystems