Supplemental Data from Multiregion Sequencing Reveals the Genetic Heterogeneity and Evolutionary History of Osteosarcoma and Matched Pulmonary Metastases

Supplementary Figure S1-S16: Figure S1: landscape of variants in all samples; Figure S2: cancer cell fraction of each mutation; Figure S3: patient screen; Figure S4: high frequency CNV in each sample; Figure S5: SNV disparity between PT and MT; Figure S6: SV features; Figure S7: gene enrichment of each gene category; Figure S8: Jaccard similarity in each patient; Figure S9: clonal status of potential driver genes; Figure S10: mutation signature enrichment in trunk, PTun and MTun; Figure S11: mutation signature enrichment in PT and MT; Figure S12: mutation spectrum in PT and MT; Figure S13: immunohistochemistry of PD-L1 and CD8 in PT and MT; Figure S14: CNV of immune regulatory genes in all samples; Figure S15: response to immune checkpoint inhibitor in patient OS06. Figure S16: The genomic microsatellite stability state in all tumor samples.