Supplementary Methods from the Pathognomonic FOXL2 C134W Mutation Alters DNA-Binding Specificity

Detailed supplemental methods of cell lines and cell culture, cell cycle synchronization and inducible expression of FOXL2, quantitative mass spectrometry analysis of induced FOXL2 expression, FOXL2 ChIP-seq analysis, RNA-seq analysis, and electrophoretic mobility shift assay.