Agrobacterium-mediated transformation of Zymoseptoria tritici v1

This protocol describes the procedure for transforming plasmids into the plant pathogenic fungus Zymoseptoria tritici by Agrobacterium tumefaciens-mediated transformation. It is based on the protocol first described by Zwiers and De Waard (2001; doi: 10.1007/s002940100216) and utilises A.tumefaciens ternary vectors developed by Sidhu et al. (2015a; doi: 10.1016/j.fgb.2015.04.015), Sidhu et al. (2015b; doi: 10.1016/j.fgb.2015.03.021) and Kilaru et al. (2015; doi: 10.1016/j.fgb.2015.03.018). Section 1 details the recipes for media used in the protocol Section 2 describes how to make competent A. tumefaciens cells Section 3 details the process of transforming A. tumefaciens competent cells with plasmid vectors Section 4 describes the process of A. tumefaciens-mediated Z. tritici transformation