Moxibustion Ameliorated Age-Related Alterations in Neurological Behavior and Gut Microbiota in Mice.

OBJECTIVE:To observe the anti-aging effects of moxibustion on age-related alterations in middle-aged mice.METHODS:Thirty, 9-month-old, male ICR mice were randomly divided into the moxibustion and control groups (N = 15). Mice in the moxibustion group were given mild moxibustion at the Guanyuan acupoint for 20 minutes every other day. After 30 treatments, neurobehavior tests, lifespan, gut microbiota composition and splenic gene expression were observed in the mice.RESULTS:Moxibustion improved the locomotor activity as well as motor function, activated the SIRT1-PPARα signaling pathway, ameliorated age-related alterations in gut microbiota, and affected the expression of genes related to energy metabolism in spleen.CONCLUSION:Moxibustion ameliorated age-related alterations in neurobehavior and gut microbiota in middle-aged mice.