SHARD: an Improved Method for Staining and Visualizing Multiplex Immunofluorescence in Optically Cleared Postmortem Human Brain Tissue

Grace A. Rosen,Daniel Kirsch,Raymond Nicks,Hunter Kelley,Rebecca Mathias,Kerry A. Cormier,Caroline A. Kubilus, Bryan Dec,Thor D. Stein,Victor E. Alvarez,Michael L. Alosco,Ann C. McKee,Bertrand R. Huber

FRONTIERS IN NEUROSCIENCE（2024）

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Key words

optical clearing,antigen retrieval,volumetric imaging,3D confocal imaging,3D confocal laser scanning microscopy,human brain banking,immunofluorescence

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