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Engineering of CRISPR-Cas PAM Recognition Using Deep Learning of Vast Evolutionary Data

Stephen Nayfach,Aadyot Bhatnagar, Andrey Novichkov, Gabriella O Estevam, Nahye Kim, Emily Hill,Jeffrey A Ruffolo, Rachel Silverstein, Joseph Gallagher,Benjamin Kleinstiver, Alexander J Meeske, Peter Cameron,Ali Madani

bioRxiv the preprint server for biology（2025）

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